Arabidopsis Ascorbate Oxidase Mutant Characterization to Determine its Physiological Role in Plant Development

Abstract

The T-DNA insertional mutant assemblies of genes of Arabidopsis thaliana are making a significant contribution to
research in plant biology since past two decades. A broad-spectrum of problem in plant biology intended at in-vivo gene
characterization for various functions were being studied by these mutant alleles. The study was undertaken to appraise
the regulatory role of ascorbate oxidase gene in plant development to see its role in reactive oxygen species. To evaluate
the functional role of ascorbate oxidase a mutant lacking AO was compared to the wild-type. For this purpose, a salk-line
homozygous for an insertion in the AO coding sequence was used and verified for the presence of the insertional
mutagenesis. Much effort needs to complete this picture, and ultimately fulfill the present-day gap in our judgement of
such potential instigator of plant responses.